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Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan
Department of Pathology, Tohoku University Hospital, Sendai, Japan
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cancer is estrogen dependent, ER immunohistochemistry is performed on pathological specimens. The results of this ER immunohistochemical analysis are considered when determining the indications for the endocrine therapies described above. As a type of
Garvan Institute of Medical Research, Sydney, NSW, Australia
St Vincent’s Clinical School, University of New South Wales, Sydney, NSW, Australia
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Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia
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Garvan Institute of Medical Research, Sydney, NSW, Australia
St Vincent’s Clinical School, University of New South Wales, Sydney, NSW, Australia
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on cell lineage rather than hormonal expression. This followed the WHO 2017 classification recommending the incorporation of transcription factor immunohistochemistry (IHC) into the routine pathological analysis of pituitary tumours ( Lloyd 2017
Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan
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Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan
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receptors are usually visualized using immunohistochemistry in formalin-fixed paraffin-embedded (FFPE) tissues. For breast cancers, immunohistochemistry of steroid hormone receptors has been used in clinical practice for the selection of specific therapies
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Ectopic adrenocorticotrophic hormone (ACTH) secretion (EAS) is a rare cause of ACTH-dependent Cushing’s syndrome (CS), most often caused by a thoracic neuroendocrine tumor (NET). Large-cell neuroendocrine carcinomas (LCNEC) with EAS are rare and usually present a more severe ACTH secretion and hypercortisolism. We report a 44-year-old non-smoker man, who presented clinical and biochemical evidence of ACTH-dependent CS. Desmopressin 10 μg i.v. produced a 157% increase in ACTH and a 25% increase in cortisol from baseline; there was no stimulation of ACTH or cortisol during the corticotropin-releasing hormone (CRH) test and no suppression with high dose dexamethasone. Pituitary MRI identified a 5 mm lesion, but inferior petrosal venous sinus sampling under desmopressin did not identify a central ACTH source. Thorax and abdominal imaging identified a left lung micronodule. Surgery confirmed a lung LCNEC with strongly positive ACTH immunohistochemistry (IHC) in the primary and lymph node metastasis. The patient was in CS remission after surgery and adjuvant chemotherapy but developed a recurrence 9.5 years later, with LCNEC pulmonary left hilar metastases, ectopic CS, and positive ACTH IHC. This is the first report of LCNEC, with morphologic feature of carcinoid tumor of the lung with ectopic ACTH stimulated by desmopressin. Long delay prior to metastatic recurrence indicates relatively indolent NET. This case report indicates that response to desmopressin, which usually occurs in Cushing’s disease or benign NETs, can occur in malignant LCNEC.
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Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Endocrinology Unit, Milan, Italy
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related to cell-cell adhesion, cadherin signaling, Wnt signaling, and cell proliferation ( Bujko et al. 2019 ). By immunohistochemistry analysis, the cell-cycle inhibitor p27 was found to be significantly reduced, whereas the chaperone HSP90 and the
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Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
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SDHB immunohistochemistry results. This study was approved by the Ethical Review Board of Ghent University Hospital (BC-10125) and conducted in accordance with the Declaration of Helsinki. All statistical procedures were performed using Statistical
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East Anglian Medical Genetics Service, Cambridge University Hospital NHS Foundation Trust, Cambridge, UK
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Department of Endocrinology, Cambridge University Hospital NHS Foundation Trust, Cambridge, UK
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. Tumour immunohistochemistry Immunohistochemical staining was performed with monoclonal mouse anti-human Ki-67 antibody (clone MIB-1, M7240; dilution 1:100; Agilent Technologies) in a Leica BOND-III IHC autostainer, with antigen retrieval performed at pH
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(×40). - Immunohistochemistry – tumour cells express melan A and calretinin; no expression of chromogranin or reaction with an antibody cocktail to cytokeratin. Where the percentage of pleomorphic cells is increased, a higher proportion of tumour cells
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Oxford NIHR Biomedical Research Centre, Oxford University Hospitals Trust, Oxford, UK
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Oxford NIHR Biomedical Research Centre, Oxford University Hospitals Trust, Oxford, UK
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transcriptional repressor 1; hMLH1, human MutL homolog 1; 5hmC, 5’ hydroxymethylcytosine; HOPX, HOP homeobox; IGF2, insulin-like growth factor 2; IHC, immunohistochemistry; INA, internexin neuronal intermediate filament protein alpha/alpha-internexin; INS
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examination revealed pituitary metastatic adenocarcinoma. Immunohistochemistry staining showed strong positivity for CK7, CKAE1/ AE3, and EMA and negativity for CK20, ER, PR, mammoglobin, vimentin, and GFAT. Thereafter, she experienced left upper limb pain
